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Science Question #1
Passage 1
Various methods are used to estimate the number of microorganisms in a culture. One method utilizes a spectrophotometer to measure the density of organisms in a broth medium. Density indicates the quantity of organisms in a given volume.
In this method, a beam of light is passed through the broth medium. The density of microorganisms in the broth is inversely proportional to the intensity of the light that passes through the broth. A meter is used to indicate the percentage of light transmitted through the medium.
The spectrophotometric data are used to produce a graph, such as the one below, that relates optical density to cell number.
In Figure 1, how does the growth of microorganisms in setup A compare to the growth in setup B?
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